| 中文名稱 | 英文名稱 | 發明人 | 專利別 | 專利國別 | 中文摘要 | 英文摘要 |
| 人類間質幹細胞體外快速增生之佐劑、體外快速擴增人類間質幹細胞之方法、體外快速擴增人類間質幹細胞以獲取生長因子的方法及其用途 | ADJUVANT FOR RAPID PROLIFERATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO, METHOD FOR RAPID PROLIFERATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO, METHOD FOR GROWTH FACTOR HARVESTED FROM RAPID PROLIFERATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO AND USE THEREOF | 孫立易馮清榮鄭敬楓李典錕 | 發明 |
台灣 日本 大陸 美國 |
本發明關於一種人類間質幹細胞體外快速增生佐劑，用以解決習知人類間質幹細胞培養時細胞擴增效率不佳之問題。本發明之人類間質幹細胞培養添加佐劑包括至少一種抗氧化劑、一第二型纖維母細胞生長因子(FGF-2)。藉此，將前述佐劑加入包含人類間質幹細胞之培養基中，於常氧環境(約21%氧分壓)培養後，出現細胞快速分裂、細胞週期合成期(S phase)比例提高、減少老化及提高分化潛能等現象，使本發明不僅可快速地擴增人類間質幹細胞並獲取生長因子，且仍可保有幹細胞多功能性之特徵，作為人類間質幹細胞之培養及擴增之用途。 | An adjuvant for rapid proliferation of human mesenchymal stem cells in vitro is provided to overcome the problem of low cell amplification efficiency of human mesenchymal stem cells in a culture process. The adjuvant added for the culture of human mesenchymal stem cells includes at least one antioxidant, and a basic fibroblast growth factor (FGF-2). The adjuvant is added into a medium containing the human mesenchymal stem cells, and the culture takes place in a normal oxygen environment (21% oxygen tension), and the cells are divided rapidly, and the cell cycle at synthesis phase (S phase) percentage is increased to reduce ageing and improve differentiationpotential. The adjuvant not only amplifies human mesenchymal stem cells rapidly to harvest the growth factor, but also maintains the characteristics of the multifunction of stem cells for the purposes of culturing and amplifying the human mesenchymal stem cells. |
