| 中文名稱 | 英文名稱 | 發明人 | 專利別 | 專利國別 | 中文摘要 | 英文摘要 |
| 體外快速切碎脂肪組織以分離活細胞之方法 | Method for rapid mincing of adipose tissues to isolate live cells in vitro |
孫立易李典錕馮清榮張耀仁 |
發明 |
台灣 大陸 美國 |
本發明關於一種體外快速切碎脂肪組織以分離活細胞之方法，以解決習知以手持刀具切碎脂肪組織作為分離脂肪組織中活細胞之前置步驟，僅能分離出少量活細胞且具較低的細胞存活率之缺點。本發明之方法包括：提供一脂肪組織；利用一切割裝置將前述脂肪組織均質切碎；於前述已切碎之脂肪組織中加入一試劑進行水解；進一步離心分離；移除上清液，以獲取一細胞沉澱物。藉此，可縮短切碎脂肪組織之時間，且避免刀具重複使用造成汙染，並提高自每單位重量之脂肪組織獲得活細胞數，且不降低細胞存活率，作為分離脂肪組織活細胞之用途。 | A method for rapid mincing of adipose tissues to isolate live cells in vitro is disclosed to overcome the drawbacks of a lead procedure of isolating live cells by holding a knife to mince adipose tissues, which can only isolate a small number of live cells and obtain low cell viability. The method includes: providing an adipose tissue; mincing the adipose tissue homogeneously by a mincing device; adding a reagent into the minced adipose tissue to perform hydrolysis; performing centrifuge and isolation; and removing a supernatant to obtain a cell pellet. Therefore, the time of mincing adipose tissues can be shortened, and contaminations caused by repeated use of the knife can be avoided. The method can be used for isolating live cells of adipose tissues to improve the number of live cells per unit weight of adipose tissues without reducing the cell viability. |
